@article{2019Alarcon,
  title = {Self-Assembled Phage-Based Colloids for High Localized Enzymatic Activity},
  journal = {ACS Nano},
  abstract = {Catalytically active colloids are model systems for chemical motors and active matter. It is desirable to replace the inorganic catalysts and the toxic fuels that are often used, with biocompatible enzymatic reactions. However, compared to inorganic catalysts, enzyme-coated  colloids  tend  to  exhibit  less  activity.  Here,  we  show  that  the  self-assembly  of genetically  engineered  M13  bacteriophages  that  bind  enzymes  to  magnetic  beads ensures high and localized enzymatic activity. These phage-decorated colloids provide a proteinaceous environment for directed enzyme immobilization. The magnetic properties of the colloidal carrier particle permit repeated enzyme recovery from a reaction solution, while the enzymatic activity is retained. Moreover, localizing the phage-based construct with a magnetic field in a microcontainer allows the enzyme-phage-colloids to function as an  enzymatic  micropump,  where  the  enzymatic  reaction  generates  a  fluid  flow.  This system  shows  the  fastest  fluid  flow  reported  to  date  by  a  biocompatible  enzymatic micropump.  In  addition,  it  is  functional  in  complex  media  including  blood  where  the enzyme driven micropump can be powered at the physiological blood-urea concentration.},
  volume = {13},
  pages = {5810–5815},
  month = mar,
  year = {2019},
  author = {Alarcon-Correa, Mariana and Guenther, Jan-Philipp and Troll, Jonas and Kadiri, Vincent Mauricio and Bill, Joachim and Fischer, Peer and Rothenstein, Dirk},
  doi = {https://doi.org/10.1021/acsnano.9b01408},
  url = {https://pubs.acs.org/doi/10.1021/acsnano.9b01408},
  month_numeric = {3}
}