HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract.
2007
Article
ei
A simple HPLC method was developed for determination of quercitrin and isoquercitrin in rat plasma. Reversed-phase HPLC was employed for the quantitative analysis using kaempferol-3-O--d-glucopyranoside-7-O--l-rhamnoside as an internal standard. Following extraction from the plasma samples with ethyl acetate-isopropanol (95:5, v/v), these two compounds were successfully separated on a Luna C18 column (250 × 4.6 mm, 5 µm) with isocratic elution of acetonitrile-0.5% aqueous acetic acid (17:83, v/v) as the mobile phase. The flow-rate was set at 1 mL/min and the eluent was detected at 350 nm for both quercitrin and isoquercitrin. The method was linear over the studied ranges of 50-6000 and 50-5000 ng/mL for quercitrin and isoquercitrin, respectively. The intra- and inter-day precisions of the analysis were better than 13.1 and 13.2%, respectively. The lower limits of quantitation for quercitrin and isoquercitrin in plasma were both of 50 ng/mL. The mean extraction recoveries were 73 and 61% for quercitrin and i soquercitrin, respectively. The validated method was successfully applied to pharmacokinetic studies of the two analytes in rat plasma after the oral administration of Hypericum japonicum thunb. ethanol extract.
Author(s): | Li, J. and Wang, WZ. and Zhang, L. and Chen, HX. and Bi, SK. |
Journal: | Biomedical Chromatography |
Volume: | 22 |
Number (issue): | 4 |
Pages: | 374-378 |
Year: | 2007 |
Month: | December |
Day: | 5 |
Department(s): | Empirical Inference |
Bibtex Type: | Article (article) |
DOI: | 10.1002/bmc.942 |
Language: | en |
Organization: | Max-Planck-Gesellschaft |
School: | Biologische Kybernetik |
Links: |
Web
|
BibTex @article{6169, title = {HPLC analysis and pharmacokinetic study of quercitrin and isoquercitrin in rat plasma after administration of Hypericum japonicum thunb. extract.}, author = {Li, J. and Wang, WZ. and Zhang, L. and Chen, HX. and Bi, SK.}, journal = {Biomedical Chromatography}, volume = {22}, number = {4}, pages = {374-378}, organization = {Max-Planck-Gesellschaft}, school = {Biologische Kybernetik}, month = dec, year = {2007}, doi = {10.1002/bmc.942}, month_numeric = {12} } |